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Protein Enzyme-Linked Immunosorbent Assay (ELISA) Tests for allergen proteins. The Neogen Allergen Protein ELISA Kits utilise a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The colour development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution. -
The complete 3M Sample Handling Collection. Better testing begins with better samples – and better samples are obtained when you use the best collection tools. 3M™ Sample Collection, Preparation and Processing solutions provide quality products for every step of your process. Convenient, ready-to-use products. Consistent, reliable results. Every time. Choosing a sampling sponge. When determining which sampling sponge to use, it’s important to consider certain factors in your testing process:- Bacteria
- Biocides
- Environment
- Strength
- Consistency
- Temperature
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Select QC Rules and make meaningful QC decisions IAMQC Expert, from Technopath Manufacturing Ltd, is an interactive system that helps front line laboratory staff select QC rules, reduce unnecessary repeats and make meaningful QC decisions. IAMQC Expert allows the end-user to monitor method performance relative to clinical requirements and focus on the tests that require their attention. Times and technologies are changing rapidly. Instruments and methodologies are more accurate, precise and stable than they were a decade ago. Most laboratories have adopted these new technical advances, but few have modified their QC processes to match. Many laboratories are still using a 1-2s rule as recommended by Levey and Jennings in 1951. Technopath Manufacturing Ltd have designed a QC system that will alert users to significant changes and not generate QC flags when the system is operating safely within acceptable limits. The system compares method performance to defined quality requirements (rather than to last month’s data) and recommends QC strategies that will warn users when QC data points exceed acceptable performance - with a minimal number of false flags. In the design of our QC system we “balance” the quality control system to meet the changing performance and stability of the analytical system. -
Internal Quality Control Software from Technopath Manufacturing Ltd. IAMQC Daily is a comprehensive Internal Quality Control software that applies Westgard and/or any user-defined QC rules to individual QC results. The software automatically builds interactive Levey-Jennings charts and tables and provides summary and detailed customised reports to the end user. IAMQC Daily integrates with Microsoft Excel to produce customised electronic reports. The system also allows the import of pre-defined templates, resulting in instant system setups. Users can create audit trails, action logs and summary reports at the click of a button. IAMQC Daily comprises a centralised program that facilitates the analysis of multiple QC materials, across numerous departments in a laboratory setting. The system can also be integrated with IAMQC Peer to satisfy both Internal and External QC requirements. IAMQC Daily offers a centralised review of all QC data from all laboratories/instruments. Central administrator access facilitates managers to review QC performance at multiple facilities – no need to visit each laboratory site. Closer monitoring of QC from remote locations without additional costs provides a flexible option for managing the inter and intra-lab performance. The software works on an ‘open’ platform that allows the end user to add all types of control material from a range of laboratories. Both IAMQC Daily and Expert can run using an internet OR intranet connection. Each PC license allows the user to manage an unlimited number of departments, control materials (not limited to Technopath materials) and instruments. An unlimited number of user logins can be added to the system at any stage. An administrator module can also manage user logins, customising the functionality that is available to each user. All data can also be filtered using the same logic to apply a user-friendly atmosphere and save time scrolling through data. -
Innovative, real-time, Peer Comparison Software from Technopath Manufacturing Ltd. The web based system facilitates laboratories testing the same lot number of control material to access valuable information from their colleagues through peer comparison. The reports that are generated in IAMQC Peer compare the accuracy and precision of analytical processes between laboratories and peer groups. This information can be extremely valuable, indicating the user’s performance relative to their peer group and also providing powerful troubleshooting tools when attempting to resolve potential problems. To participate in IAMQC Peer, each individual laboratory submits their individual results or summary statistics (mean, standard deviation, and number of data points) to the central database maintained by Technopath Manufacturing Ltd. Laboratories data may be submitted manually on-line or, alternatively, captured by one of our many interfacing options. The information provided by IAMQC Peer can be used on a monthly basis to evaluate how well lab’s methods are operating, relative to the overall peer group. Users can also look at this peer data in real-time, interactive, tables online, when they are investigating a potential problem with accuracy or precision for an individual method. -
Lateral flow devices for specific allergen detection Streamline your process and simplify your allergen testing with 3Neogen Allergen Protein Rapid Tests – qualitative immunochromatographic assays for rapid in-plant monitoring of specific food allergens. Designed for accurate detection of processed and unprocessed allergen proteins, these fast, easy tests can be used for clean-in-place (CIP) final rinse water, environmental swab samples, raw ingredients and finished food products. We’ve taken the hassle out of allergen testing. You see the results. -
Colifast Field Kit is a portable tool for field detection of coliform bacteria in water. The main method of the kit is a rapid method that provides an answer as early as 15 minutes, 2 hours at the latest. In addition, the kit includes methods for a quantitative MPN (Most Probable Number) method and presence/absence results. The procedure is very easy to perform, and requires no laboratory skills. Colifast Field Kit is delivered in a robust and portable metal case, and all equipment required for the water analysis is included. The Colifast Micro Detector (CMD) is used in combination with a growth medium for specific detection of target bacteria. Combined, they provide a simple instrumental analysis and rapid results that quantify the amount of coliform and thermotolerant bacteria in water. The method is based upon the patented technology of Colifast AS. The system detects down to 1 target bacterium per sample volume. Method The Colifast technology is based on a chemical reaction between a substrate in the growth medium and enzymes produced by the coliform bacteria. The bacterial enzyme β-D-galactosidase hydrolyses the substrate 4-methylumbelliferyl-β-D-galactoside, which results in the release of the fluorescent product 4-methylumbelliferone (MU). In addition to substrate, activators and growth factors, the medium comprises inhibitors that prevent the growth of non-coliform bacteria. The results are measured in fluorescent units. An increase in the number of bacteria corresponds to an increased amount of β-D-galactosidase (enzyme). The subsequent increase in the amount of MU (fluorescent product) results in a higher value on the instrument (Colifast Micro Detector). The incubation temperature selects for thermotolerant coliforms at 44 °C and total coliforms at 37 °C. -
The Colifast At-Line Monitor – CALM is a fully automated early warning system for monitoring of coliforms and Pseudomonas aeruginosa in water. Detection time ranges from 2 to 12 hours. The technology is time-saving, cost-reducing, and environmentally friendly. Examples of CALM applications are monitoring of raw water, in-process water, waste water and recreational water. No laboratory facilities and minimal skills in microbiology are required to operate the instrument. The Colifast CALM is easily connected to the system computer or LAN interface. The patented Colifast growth media are supplied in Pre-filled Multi Well Trays™ and the flexibility of the system makes it easy to adapt to different applications. The CALM can perform parallel analysis of different target organisms. Depending on method and test frequency, the CALM needs monthly or weekly refilling of reagents. Technology The CALM instrument detects and quantifies E. coli, fecal or total coliform bacteria in water by fluorometric monitoring of β-glucuronidase activity (E. coli) or β-galactosidase activity (fecal and total coliforms). The detection of P. aeruginosa is based on substrate hydrolysis of aminopeptidase enzymes present in this bacterium. The Colifast technology is comprised of unique media formulations combined with instrument detection systems. The technology is covered by patents in numerous countries, with several international patents pending. Growth of other bacteria is stopped by inhibitors in the medium and by incubation of the samples at high temperature: Incubator temperatures for selection of thermotolerant coliform bacteria are 44 ° C and total coliforms / E. coli are 37 ° C. Method The instrument can perform one of two methods at a time. The Most Probable Number (MPN) method is based on the cultivation of bacteria. A certain volume is added to 4-6 wells of growth medium and the bacterial number is determined from how many of the wells that gave growth after the incubation period (11-12 hours). For the results to be as accurate as possible, it is therefore important that the samples taken on a given source usually give a mixture of positive and negative results. The rapid method is also a quantitative method, which is based on the enzyme activity of the bacteria present in the water sample. It provides response after 75-115 min and is suitable for water sources where major contamination events can occur. The CALM is a flexible instrument. This means that the instrument can be adapted to different locations with different occurrence of bacteria. If a source has an average bacterial count of 500 cfu / 100ml, 0.2 ml will usually give a positive test. By injecting about half of this volume, you will often get both positive and negative samples. If the source has lower concentrations of bacteria, the media concentration can be changed so that the sample volume can be as high as 10 ml. In this way, we ensure good results from the CALM instrument with optimal concentration of media after addition of sample and a sample volume appropriate for the source being measured. -
Rapid, Automated and Remote Microbial Testing for Water and Food Safety Colifast ALARM™ is an electronic instrument for detecting indicator bacteria in drinking water using patented Colifast technology. 100 ml water samples are automatically collected at programmed intervals and analyzed for total coliforms, thermotolerant coliform bacteria or E. coli. In addition to performing rapid, online microbial water analysis, Colifast ALARM measures water turbidity. The system can automatically send results to control rooms / operators via LAN, digital signals or via mobile networks (SMS). The system can detect down to 1 cfu / 100 mL, and results are achieved within 6-14 / 15 hours. Technology The main components of Colifast ALARM are the incubator chamber, an injection pump system for fluid handling, and a detector system that includes wavelength-specific emitters combined with a spectrometer. Detection of down to 1 viable target bacteria is based on bacterial growth, group-specific enzyme activity and measured concentrations of a fluorescent product (ppb MU). An increase in the number of target bacteria means an increase in the amount of β-D-glucuronidase (E. coli-specific enzyme). The enzyme hydrolyses the growth medium substrate to MU (the fluorescent product) and β-D-glucuronic acid. Increased MU release gives increased fluorescence signal on the Colifast ALARM. Colifast growth medium contains inhibitors to prevent growth of non-coliforms. Colifast ALARM is verified by EPA's ETV program. -
Enteral Syringe Driver For the application of enteral nutrition only. Deliver small volumes of enteral feed, accurately and safely, using enteral ENFit syringes. -
Colostrum Gold Kit Kit for the collection and administration of Colostrum available with 1ml or 3ml syringes. -
ENPLUG™ The ENPLUG™ is designed for emergency use to keep stomas from closing when a gastrostomy device has fallen out. The ENPLUG packs are available in 2 different lengths and various French sizes in case the stoma has already started to close and the current French gauge tube no longer fits the stoma.
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