Shop

Phenotype MicroArrays for Mammalian Cells Using Biolog’s Phenotype MicroArray™ Technology, any type of cell can be characterised for metabolically related phenotypes in over 1,000 assays in a simple, rapid, and cost-effective manner. By measuring a cell’s metabolic activity, including responses to a genetic alteration or chemical stimuli, this integrated system of cellular assays, instrumentation, and bioinformatic software reveals unique and insightful information on metabolic pathway activities, and cellular sensitivity to nutrients, hormones, cytokines, anti-cancer agents, and ions. By measuring energy output of cells, the technology also provides new assays for understanding mitochondrial function and toxicity.  Phenotype MicroArrays enable insight and discovery to expedite scientific publications. This is accomplished using 96 well microplates, pre-loaded with hundreds of KEGG pathway-matched metabolic substrates, and metabolic effectors. Phenotype MicroArrays are a proven method of cellular screening that is beneficial in a wide range of research applications:

• Genotype/phenotype studies
• Cell line characterisation and QC
• Cellular metabolism, metabolic disorders, nutrition
• Cell energetics growth and death
• Hormone effects on cells
• Metabolic reprogramming in cancer, Warburg effect, anti-cancer drug sensitivity
• Mitochondrial toxicology in chemical/drug screens
• Stem cells and differentiation
• Cell line and bio process development

Analysing Mitochondria at Higher Resolution with MitoPlate™ Technology Analysing Mitochondria at higher resolution with Biolog Technology MitoPlates™ provide a powerful new research tool by allowing scientists to run preconfigured sets of 96 mitochondrial function assays in one experiment. Mitochondria can be interrogated and characterised in novel ways, looking at rates of substrate metabolism, sensitivity to drugs and other chemicals, and effects of mutations in mitochondria-related genes. Assay Principle Mitochondrial function is assayed by measuring the rates of electron flow into and through the electron transport chain from metabolic substrates that produce NAD(P)H or FADH2 such as L-malate, succinate, pyruvate, etc. Each substrate follows a different route, using different transporters to enter the mitochondria and different dehydrogenases to produce NAD(P)H or FADH2, The electrons travel from the beginning (complex 1 or 2) to the distal portion of the electron transport chain where a tetrazolium redox dye (MC) acts as a terminal electron acceptor that turns purple upon reduction. Additional MitoPlate assays probe the sensitivity of the mitochondria to a set of 22 diverse inhibitors. Investigate how mitochondria change with:

• Cell differentiation
• Cancer & Aging
• Neurological disorders
• Metabolic disorders
• Immune cell activation
• Bacterial/viral infection
• Inborn genetic defects

Microbial ID microplates from Biolog Biolog Solutions for Microbial Identification Rapidly and accurately identify nearly 3,000 species of aerobic and anaerobic bacteria, yeast, and fungi. In addition to species-level identification, Biolog’s advanced phenotypic technology provides valuable information on the functional properties of strains. Biolog’s carbon source utilisation technology identifies environmental and pathogenic microorganisms by producing a characteristic pattern or “metabolic fingerprint” from discrete test reactions performed within a 96-well microplate. Culture suspensions are tested with a panel of pre-selected assays, then incubated, read and compared to our extensive database. Biolog’s Microbial ID microplates incorporate a patented redox tetrazolium dye that changes colour as a result of cellular respiration, providing a metabolic fingerprint. Our complete solution includes instruments, consumables, databases, software.