Anaerobic Culture Media

Pre-reduced anaerobially sterilised (PRAS) plated and broth culture media

Biolog Solutions for Anaerobic Culture Media

Anaerobic organisms play critical roles in health and disease pathology of humans and animals, as well as throughout the Earth’s ecosystems. Isolating and culturing these fastidious organisms with consistent results requires specialised conditions.

Your research can benefit from our decades of experience with formulating and manufacturing special nutritive TruPRAS™ media that never comes into contact with oxygen. We provide a wide range of media for specimen collection and transport, enriched plated agar media, and enriched liquid media specifically designed for culturing even organisms that were previously thought to be unculturable.

Resources:

Resources:

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View TruPAS Media Brochure here

The Oxygen-Free Difference

From pouring to packaging, the absence of oxygen in TruPRAS media prevents the formation of harmful byproducts. When opened in oxygen-free conditions, you can be sure that oxidative damage is ruled out as a variable. And since it can be stable at room temperature and is ready to use right out of the package, oxygen-free also means no wasted time.

TruPRAS™ Media

Specimen Collection & Transport

Anaerobe Systems’ transport tubes are poured and packaged under anaerobic conditions (TruePRAS™ media). The unique process prevents oxygen contamination or damage to the culture media during the manufacturing process. Reducing agents and visual oxygen indicators help prevent oxidation when adding a sample, and will indicate the portion of the medium still anaerobic.

BIOME-Preserve is a patented, first of its kind kit for collecting, transporting, and preserving anaerobic microbiome samples such as stool. It allows for easy self-collection of a sample at home, room temperature transport for up to 5 days, and culture recovery of the majority of species present in the sample. BIOME-Preserve may also be directly frozen for long-term storage.

We can make custom media formulations – enquire today.

Product Description	Catalog Number	Tubes/Pack
Anaerobic Transport Medium – ATM	AS-911	10
Anaerobic Transport Medium Surgery Pack – ATMSP	AS-914	10
Anaerobic Tissue Transport Medium Surgery Pack – ATTMSP	AS-915	5
Liquid Dental Transport – LDT	AS-916	10
Anaerobic Tissue Transport Medium – ATTM	AS-919	5
Dental Transport Medium – DTM	AS-920	10
BIOME-Preserve Microbiome Collection Kit (for research use only)	AS-930	1

Agar Plates

Anaerobe Systems’ plated agar packs are poured and packaged under anaerobic conditions (TruePRAS™ media). Our unique process prevents oxygen contamination or damage to the culture media during the manufacturing process. Anaerobe Systems agar plates are pre-reduced and ready to use out of the package, without the need to leave them in an anaerobic environment for extended periods before inoculation.

Product Description	Catalog Number	Minimum Order	Plates/Pack
Bacteroides Bile Esculin Agar – BBE	AS-114	n/a	1
Bacteroides Bile Esculin Agar- BBE	AS-144	n/a	4
Bacteroides Bile Esculin and Laked Kanamycin Vancomycin Bi-Plate – BBE/LKV	AS-212	n/a	1
Bacteroides Bile Esculin and Laked Kanamycin Vancomycin Bi-Plate – BBE/LKV	AS-242	n/a	4
Bifidobacterium Selective Agar – BIFIDO	AS-6423	25 packs	4
Botulism Selective Media – BSM	AS-223	25 packs	4
Brain Heart Infusion Agar – BHI	AS-6426	n/a	4
Brain Heart Infusion Agar w/ Horse Blood & Taurocholate – BHIY-HT	AS-6463	n/a	4
Brucella Blood Agar – BRU	AS-111	n/a	1
Brucella Blood Agar – BRU	AS-141	n/a	4
Brucella Blood Agar – BRU – 150mm plate	AS-614	n/a	1
Campylobacter Agar – CAMPY	AS-211	100	1
CDC Anaerobe Blood Agar – CABA or CDC	AS-646	n/a	4
Chocolate Agar – CHOC	AS-214	100 packs	1
Chocolate Agar – CHOC	AS-244	25 packs	4
Clindamycin Blood Agar – CBA	AS-645	25 packs	4
Columbia Blood Agar – CA	AS-895	25 packs	4
Crystal Violet-Erythromycin Agar – CVE	AS-647	25 packs	4
Cycloserine-Cefoxitin Fructose Agar – CCFA	AS-213	n/a	1
Cycloserine-Cefoxitin Fructose Agar with Horse Blood and Taurocholate – CCFA-HT	AS-2136	n/a	1
Cycloserine-Cefoxitin Fructose Agar with Lysozyme – CCFAL	AS-2133	100 packs	1
Egg Yolk Agar – EYA	AS-511	n/a	1
Enriched Tryptic Soy Agar – ETSA	AS-548	25 packs	4
Fusobacterium Selective Agar – FSA	AS-6427	25 packs	4
Lactobacilli-MRS Agar – LMRS	AS-6429	25 packs	4
Laked Brucella Blood Agar w/ Kanamycin and Vancomycin – LKV	AS-112	n/a	1
Laked Brucella Blood Agar w/ Kanamycin and Vancomycin – LKV	AS-142	n/a	4
Mitis-Salivarius Agar with Tellurite – MSAT	AS-544	25 packs	4
MTGE Anaerobic Enrichment Agar – MTGE	AS-777	25 packs	4
Oral H2S Organism Agar w/ Lead Acetate – OHO-C	AS-6430	25 packs	4
Phenylethyl Alcohol Blood Agar – PEA	AS-113	n/a	1
Phenylethyl Alcohol Blood Agar – PEA	AS-143	n/a	4
Porphyromonas gingivalis Agar – P. GING	AS-6422	25 packs	4
Reinforced Clostridial Agar – RCA	AS-6061	25 packs	4
Tryptic Soy Agar with N-Acetylmuramic Acid – TSA-NAM	AS-6421	25 packs	4
Tryptic Soy Agar with Tween and Lecithin, 25ml fill (TSA-TL)	AS-228	n/a	2
Tryptic Soy Blood Agar – TSBA	AS-542	25 packs	4
Tryptic Soy-Serum-Bacitracin-Vancomycin Agar – TSBV	AS-648	25 packs	4
Yeast Casitone Fatty Acids Agar with Carbohydrates – YCFAC	AS-675	n/a	4
Yeast Casitone Fatty Acids Agar with Carbohydrates and Sheep Blood – YCFAC-B	AS-677	25 packs	4
Yeast Malt Extract Agar, 25ml Fill – YMEA	AS-229	n/a	2

Liquid Broth (Tubes & Bottles)

Anaerobe Systems’ broth media are poured and packaged under anaerobic conditions (TruePRAS™ media). Our unique process prevents oxygen contamination or damage to the culture media during the manufacturing process. Our broth media are pre-reduced and ready to use out of the package, without the need to leave them in an anaerobic environment for extended periods before inoculation. Tubes are sealed with Hungate style caps which contain a rubber septum, allowing for inoculation without removing the cap or exposing the broth to oxygen.

We can make custom media formulations, enquire today.

Product Description	Catalog Number	Minimum Order	Tubes/Bottles per Pack
Brain Heart Infusion Broth 250ml – BHI	AS-8722	10 Bottles	1
Brain Heart Infusion Broth 500ml – BHI	AS-8725	5 Bottles	1
Brain Heart Infusion Broth 5ml – BHI	AS-872	n/a	10
Brucella Broth – BRU-BROTH	AS-105	n/a	10
Campylobacter-Thioglycollate Broth – CAMPY-THIO	AS-807	15 Packs	10
Chopped Meat Medium 250ml – CM	AS-8112	10 Bottles	1
Chopped Meat Medium 500ml – CM	AS-8115	5 Bottles	1
Chopped Meat Medium 7ml – CM	AS-811	n/a	10
Chopped Meat Medium with Carbohydrate 250ml – CMC	AS-8232	10 Bottles	1
Chopped Meat Medium with Carbohydrate 500ml – CMC	AS-8235	5 Bottles	1
Chopped Meat Medium with Carbohydrate 7ml – CMC	AS-823	n/a	10
Chopped Meat Medium with Glucose 250ml – CMG	AS-8132	10 Bottles	1
Chopped Meat Medium with Glucose 500ml – CMG	AS-8135	5 Bottles	1
Chopped Meat Medium with Glucose 7ml – CMG	AS-813	n/a	10
Cycloserine Cefoxitin Mannitol Broth w/ Taurocholate Lysozyme Cysteine – CCMB-TAL	AS-8216	15 Packs	10
Lactobacilli-MRS Broth – LMRS	AS-609	15 Packs	10
MTGE Anaerobic Enrichment Broth 250ml – MTGE	AS-7782	10 Bottles	1
MTGE Anaerobic Enrichment Broth 500ml – MTGE	AS-7785	5 Bottles	1
MTGE Anaerobic Enrichment Broth 6ml – MTGE	AS-778	15 Packs	10
Oral Treponeme Enrichment Broth – OTEB	AS-603	n/a	10
Oral Treponeme Enrichment Broth 250ml Bottle – OTEB	AS-6032	10 Bottles	1
Oral Treponeme Enrichment Broth 500ml Bottle – OTEB	AS-6035	5 Bottles	1
Peptone Yeast Broth with Glucose 250ml – PYG	AS-8222	10 Bottles	1
Peptone Yeast Broth with Glucose 500ml – PYG	AS-8225	5 Bottles	1
Peptone Yeast Broth with Glucose 7ml – PYG	AS-822	n/a	10
Peptone Yeast Extract Broth – PY	AS-821	15 Packs	10
PRAS Dilution Blank Medium 250ml	AS-9183	10 Bottles	1
PRAS Dilution Blank Medium 500ml	AS-918-500	5 Bottles	1
PRAS Dilution Blanks, 10 mL	AS-910	n/a	10
PRAS Dilution Blanks, 18 mL	AS-918	15 Packs	10
PRAS Dilution Blanks, 3 mL	AS-917	20 Packs	10
PRAS Dilution Blanks, 7 mL	AS-907	15 Packs	10
PRAS Dilution Blanks, 9 mL	AS-908	n/a	10
PY Adonitol	AS-834	15 Packs	10
PY Amygdalin	AS-836	15 Packs	10
PY Arabinose	AS-824	15 Packs	10
PY Arginine	AS-835	15 Packs	10
PY Cellobiose	AS-837	15 Packs	10
PY DL-Threonine	AS-856	15 Packs	10
PY Dulcitol	AS-838	15 Packs	10
PY Erythritol	AS-839	15 Packs	10
PY Esculin	AS-833	15 Packs	10
PY Fructose	AS-840	15 Packs	10
PY Galactose	AS-841	15 Packs	10
PY Glycerol	AS-842	15 Packs	10
PY Inositol	AS-844	15 Packs	10
PY Inulin	AS-845	15 Packs	10
PY Lactate	AS-846	15 Packs	10
PY Lactose	AS-826	15 Packs	10
PY Maltose	AS-847	15 Packs	10
PY Mannitol	AS-848	15 Packs	10
PY Mannose	AS-849	15 Packs	10
PY Melezitose	AS-850	15 Packs	10
PY Melibiose	AS-851	15 Packs	10
PY Pyruvic Acid	AS-852	15 Packs	10
PY Raffinose	AS-853	15 Packs	10
PY Rhamnose	AS-827	15 Packs	10
PY Ribose	AS-854	15 Packs	10
PY Salicin	AS-828	15 Packs	10
PY Sorbitol	AS-855	15 Packs	10
PY Starch	AS-829	15 Packs	10
PY Sucrose	AS-830	15 Packs	10
PY Trehalose	AS-831	15 Packs	10
PY Xylan	AS-859	15 Packs	10
PY Xylose	AS-832	15 Packs	10
PYG Bile	AS-857	15 Packs	10
PYG Formate/Fumarate	AS-858	15 Packs	10
PYG Gelatin 5ml	AS-860	15 Packs	10
PYG Tween	AS-825	15 Packs	10
Reinforced Clostridial Broth 250ml Bottle – RCB	AS-6062	10 Bottles	1
Reinforced Clostridial Broth 500ml Bottle – RCB	AS-6065	5 Bottles	1
Reinforced Clostridial Broth 7ml – RCB	AS-606	15 Packs	10
Thioglycollate Broth with Hemin, Vit. K, without indicator, 5mL – THIO	AS-805	n/a	10
Thioglycollate Broth with Hemin, Vit. K, without indicator, 7 mL – THIO	AS-801	n/a	10
Yeast Casitone Fatty Acids Broth with Carbohydrates 250ml Bottle – YCFAC Broth	AS-6802	10 Bottles	1
Yeast Casitone Fatty Acids Broth with Carbohydrates 500ml Bottle – YCFAC Broth	AS-6805	5 Bottles	1
Yeast Casitone Fatty Acids Broth with Carbohydrates 7ml – YCFAC Broth	AS-680	n/a	10

Clinical Isolation & ID

Anaerobe Systems manufactures a wide variety of enriched, selective, and differential culture media to aid in the isolation and identification of anaerobic infections. Anaerobe transport media can be used to keep organisms in samples alive and viable during transport from collection to the lab, and are available in sterile surgery packs.

Anaerobe Systems’ culture & transport media are sterilised, poured, and packaged under anaerobic conditions (TruePRAS™ media). There is no chance of oxygen contamination or damage any time during the manufacturing process. Our plates are pre-reduced and ready to use out of the package, without the need to leave them in an anaerobic environment for extended periods before inoculation.

Product Description	Format	Application	Selective	Differential	Enrichment	Transport
Anaerobe Chambers	n/a	Anaerobic chamber maintains strict anaerobic conditions with operator access for manipulating and growing organisms	X	X	X	X
Anaerobic Identification	n/a	Various antibiotic potency discs and biochemical reagents available for spot testing and ID		X
Anaerobic Transport Media	Tube	Transport, preserve, and enhance recovery of anaerobic specimens				X
Bacteroides Bile Esculin Agar – BBE	Plate	Selective for Bacteroides, differentiates B. fragilis group	X	X
BBE/LKV Bi-Plate	Plate	Split plate with BBE & LKV media to save space	X	X
Brain Heart Infusion Broth – BHI	Broth	General enrichment broth for growth & enumeration of broad spectrum anaerobes			X
Brucella Blood Agar – BRU	Plate	General enrichment blood agar for growing wide range of anaerobes. Contains vitmain K and hemin.			X
Brucella Blood Agar – BRU (150mm)	Plate	Oversized BRU plate for strip based antimicrobial susceptibility testing. Uniform agar depth across plate and larger area for more strips			X
Campylobacter Agar – CAMPY	Plate	Selective for Campylobacter from fecal/rectal swabs	X
Chopped Meat Medium with Carbohydrate- CMC	Broth	Enrichment broth with ground beef. Promotes toxin production. Can be used as freezer stock.			X
Cycloserine Cefoxitin Fructose Agar with Horse Blood & Taurocholate – CCFA-HT	Plate	Selects for C. difficile with taurocholate to enhance spore germination	X
Cycloserine Cefoxitin Fructose Agar – CCFA	Plate	Selective for C. difficile, colonies turn yellow	X	X
Cycloserine Cefoxitin Mannitol Broth w/ Taurocholate & Lysozyme- CCMB-TAL	Broth	Selective broth for C. difficile with color indicator for positive growth	X	X
Egg Yolk Agar – EYA	Plate	Differential plate shows lipase and lecithinase activity of microorganisms		X
Laked Blood Agar with Kanamycin & Vancomycin – LKV	Plate	Selective for gram negative obligate anaerobes. Inhibits gram positive organisms.	X	X
Phenylethyl Alcohol Blood Agar – PEA	Plate	Selective media inhibits facultative gram negative rods and inhibits swarming organisms. Black pigmentation of some organisms can be observed	x	x
Thioglycolate Broth – THIO	Broth	Enriched broth medium with small quantity of agar added to slow oxygen diffusion and a marble chip added as a pH buffer			x

Clostridium difficile

Clostridium difficile, commonly referred to as C. diff, is a prevalent and infectious anaerobic organism associated with diahrrea. Clostridium difficile is one of the most oxygen sensitive anaerobes, and although it is a spore former it can be difficult to culture. Anaerobe Systems manufactures a range of specialty media for growing and isolating Clostridium difficile.

Anaerobe Systems’ culture & transport media ares, poured, and packaged under anaerobic conditions (TruPRAS™). There is no chance of oxygen contamination or damage any time during the manufacturing process. The plates are pre-reduced and ready to use out of the package, without the need to leave them in an anaerobic environment for extended periods before inoculation.

Product Description	Format	Application	General Enrichment	Selective / Differential	Spore Germination	Transport
Anaerobic Chambers	n/a	Anaerobic chamber maintains strict anaerobic conditions with operator access for manipulating and growing organisms	x	x	x	x
Anaerobic Transport Media	Tube	Transport, preserve, and enhance recovery of anaerobic specimens				x
Brain Heart Infusion Agar with Horse Blood & Taurocholate – BHIY-HT	Plate	Non-selective enrichment plate promotes spore germination and growth. Used as a recovery medium for enumeration per ASTM E2839-11	x		x
Brucella Blood Agar – BRU	Plate	General enrichment blood agar for growing wide range of anaerobes	x
CDC Anaerobic Blood Agar – CDC or CABA	Plate	General enrichment blood agar. Used for sporulation per ASTM E2839-11	x
Chopped Meat Medium with Carbohydrate – CMC	Broth	Enrichment broth with ground beef. Promotes toxin production. Can be used as freezer stock.	x
Cycloserine Cefoxitin Fructose Agar with Horse Blood & Taurocholate – CCFA-HT	Plate	Selective media for C. difficile with taurocholate to enhance spore germination.		x	x
Cycloserine Cefoxitin Fructose Agar – CCFA	Plate	Selective for C. difficile, colonies and media turn yellow		x
Cycloserine Cefoxitin Mannitol Broth w/ Taurocholate & Lysozyme- CCMB-TAL	Broth	Selective broth for C. difficile with color indicator for positive growth		x	x
Reinforced Clostridial Broth	Broth	Enrichment broth for use in rehydrating lyophilized organisms per ASTM E2839-11	x

Microbiome and Research

Anaerobe Systems manufactures a wide variety of highly enriched, selective, and differential culture media for in growth and isolation of organisms found in the human microbiome. The unique TruePRAS™ manufacturing process is ideally suited for cultivating fastidious and difficult to culture anaerobic organisms. Anaerobe transport media can be used to keep organisms in samples alive and viable during transport from collection to the lab.

Anaerobe Systems’ culture & transport media are poured and packaged under anaerobic conditions (TruePRAS media). There is no chance of oxygen contamination or damage any time during the manufacturing process. Our plates are pre-reduced and ready to use out of the package, without the need to leave them in an anaerobic environment for extended periods before inoculation.

Product Description	Format	Application	Skin	Oral	Gut	Vaginal	Un-Culturable
Anaerobic Transport Media	Tube	Transport, preserve, and enhance recovery of anaerobic bacteria	X	X	X	X	X
BIOME-Preserve Microbiota Collection Kit (for research use only)	Tube	Individual kit for at-home collection of stool samples, facilitating room temperature transport to lab for up to 120 hours. Preserves species for culture recovery.		X	X		X
Bacteroides Bile Esculin Agar – BBE	Plate	Selects for Bacteroides, differentiates B. fragilis group		X	X	X
Bifidobacterium Selective Agar – BIFIDO	Plate	Selects for Bifidobacterium species		X	X	X
Brain Heart Infusion w/ Horse Blood & Taurocholate – BHIY-HT	Plate	Enriched medium which promotes spore germination			X
Brucella Blood Agar – BRU	Plate	General enrichment blood agar for growing wide range of anaerobes	X	X	X	X
Chocolate Agar – CHOC	Plate	Enriched media with supplement VX (nicotinamide adenine dinucleotide/NAD)	X	X	X	X	X
Cycloserine Cefoxitin Fructose Agar – CCFA-HT	Plate	Selects for C. difficile and enhances spore germination			X
Crystal Violet Erythromycin Agar – CVE	Plate	Selective for Fusobacterium and differential for F. nucleatum		X
Laked Blood Agar with Kanamycin & Vancomycin – LKV	Plate	Selective for gram negative obligate anaerobes. Inhibits gram positive organisms.	X	X	X	X
Lactobacilli MRS Agar – LMRS	Plate	Mildly selective for Lactobacillus			X	X
Mitis Salivarius Agar with Tellurite – MSAT	Plate	Selective for oral Streptococcus & Enterococcus		X
MTGE Anaerobe Enrichment Agar – MTGE	Plate	Highly enriched agar with short-chain fatty acids & serum. More enriched than BRU	X	X	X	X	X
Oral H2S Organism with Lead Acetate Agar – OHO-C	Plate	Identifies & differentiates hydrogen sulfide producing bacteria	X	X	X	X
Porphyromonas Gingivalis Agar – P.Ging	Plate	Enriched and selective for Porphyromonas gingivalis		X
Phenylethyl Alcohol Blood Agar – PEA	Plate	Selective media inhibits facultative gram negative rods and inhibits swarming organisms	X	X	X	X
Yeast Casitone Fatty Acid with Carbohydrate Agar – YCFAC	Plate	Highly enriched media with short chain fatty acids, vitamin mix, and carbohydrate	X	X	X	X	X
Yeast Casitone Fatty Acid with Carbohydrate Broth – YCFAC	Plate	Highly enriched media with short chain fatty acids, vitamin mix, and carbohydrate	X	X	X	X	X
Yeast Casitone Fatty Acid with Carbohydrate and Blood Agar – YCFAC +B	Plate	Highly enriched media with short chain fatty acids, vitamin mix, and carbohydrate. Enriched with sheep blood.	X	X	X	X	X
Anaerobe Chambers	n/a	Anaerobic chamber maintains strict anaerobic conditions with operator access for manipulating and growing organisms	X	X	X	X	X
Brain Heart Infusion Broth – BHI	Broth	General enrichment broth for growth & enumeration of broad spectrum anaerobes	X	X	X	X
Cycloserine Cefoxitin Mannitol Broth w/ Taurocholate & Lysozyme – CCMB-TAL	Broth	Selective broth for C. difficile with color indicator for positive growth			X
Chopped Meat Medium with Carbohydrate – CMC	Broth	Enrichment broth with ground beef. Promotes toxin production.	X	X	X	X
Dilution Blanks	Broth	Non-nutritive buffered mineral salt solution used in dilutions for viable organism counts.	X	X	X	X	X
Lactobacilli MRS Broth – LMRS	Broth	Mildly selective for Lactobacillus
MTGE Anaerobe Enrichment Broth – MTGE	Broth	Highly enriched agar with short-chain fatty acids & serum. More enriched than BRU	X	X	X	X	X
Oral Treponeme Enrichment Broth – OTEB	Broth	Enriched broth with short-chain fatty acids & serum to promote growth of Treponema spp.		X			X

Bagged Media

TruPRAS Bagged Media
GMP‑Ready, Large‑Volume Anaerobic Culture Media for Scalable Bioprocessing
Anaerobic organisms play critical roles in health and disease pathology of humans and animals. Isolating and culturing these fastidious organisms with consistent results requires specialized conditions. For scaling up biomanufacturing, it’s especially critical to have consistent conditions to support growth at large volumes.

The TruPAS broth media in all formats are poured and packaged under TruPRAS™ conditions, which prevents oxygen contamination or damage during manufacturing.

What Makes TruPRAS Bagged Media Different?

<Pre‑Reduced and Anaerobically Sterilised –Manufactured in a strictly anaerobic environment, preventing oxidative damage and ensuring media supports obligate anaerobe growth right out of the bag.
Large‑Volume Capacity – Flexible bag packaging accommodates higher culture volumes for bioprocessing and manufacturing environments.
Closed‑System Format – Minimises manual handling and contamination risk, compatible with standard connectors for aseptic transfers and bioreactor integration.
GMP‑Ready Manufacturing – Produced under sterile conditions ready for bioprocessing.

Consult with a Technical Specialist

Whether you’re specifying equipment for a new lab build-out or need support with an existing installation, our sector specialists are ready to help.

Ireland: +353 (0)61-335844 UK: +44 (0)28 30833808 Email: [email protected]

Anaerobic Culture Media

Anaerobic Culture Media

Resources:

Resources:

The Oxygen-Free Difference

TruPRAS™ Media

Specimen Collection & Transport

Agar Plates

Liquid Broth (Tubes & Bottles)

Clinical Isolation & ID

Clostridium difficile

Microbiome and Research

Bagged Media

Biolog ID Station^™

Biolog Odin^™

Consult with a Technical Specialist

Anaerobic Culture Media

Anaerobic Culture Media

Resources:

Resources:

The Oxygen-Free Difference

TruPRAS™ Media

Related Products

Consult with a Technical Specialist