Phenotype MicroArray™ Technology for Microbial Cells
Biolog’s Phenotype MicroArray™ Technology enables researchers to characterize cells in up to 1,920 assays and evaluate cell changes under thousands of culture conditions and physiological states in a simple, rapid, efficient and cost-effective manner. By measuring a cell’s response to a genetic or environmental alteration, this integrated system of cellular assays, instrumentation, and bioinformatics software reveals invaluable information to speed insight and discovery and expedite scientific publication. Phenotype MicroArrays (PMs) have broad applicability for genotype-phenotype characterization as well as for determining optimal conditions for cellular growth, sporulation and germination, production of secondary metabolites,or enzymatic activities in cell lines.
Phenotype MicroArrays are a proven method of cellular screening that is extremely beneficial in a wide range of research applications:
Discovering effects of loss or gain of gene function.
Measuring/documenting changes in cell metabolism over time or under different environmental conditions.
Improvement and QC of phenotypic stability of cell lines.
Improved efficacy in the production of compounds in biological fermentation processes.
Evaluation of new drug/antibiotic candidates in toxicological profiling and mode of action studies.
Biolog PhenoType MicroArray Technology Overview from Biolog on Vimeo
The OmniLog PM system includes software for analysis of Biolog Phenotype MicroArray panels. The OmniLog II Combo System has additional software and a database for identification of aerobic bacteria using Biolog’s Gen III MicroPlates. The OmniLog II Combo Plus System adds the capability for identifying anaerobes, yeast and filamentous fungi. Each system includes the OmniLog instrument, software as indicated, multimedia computer, LCD flat panel monitor, 8-channel electronic pipettor, turbidimeter, user guide, training and 1-year warranty. The OmniLog II Combo system also include the MicroStation™ MicroPlate reader and a printer. Organism databases (for microbial identification), consumables and other accessories are purchased separately.
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Biolog OmniLog® PM System |
Specifications Size: 21 in x 32 in x 23 in (53 cm x 81 cm x 58 cm) Power: 100 to 240 volts, 50 to 60 Hz Operating Temp Range: 18˚ to 28˚C Operating Humidity Range: 20% to 80% non-condensing Incubation Temperature Range: 22˚ to 45˚C Temp Consistency: ±2˚C in the tray chamber Incubation Humidity Range: Ambient Test Capacity: 50 microplates Data Capture: CCD images of each microplate every 15 minutes Temperature Control: Input of set temperature by external computer Temperature Indication: Output to external computer and 7 segment display Monitor: LCD flat panel, 17 in viewable image size |
Biolog PM Plate
Phenotype MicroArrays are pre-configured 96 well plates containing different classes of chemical compounds designed to test for the presence or absence of specific cellular phenotypes. There are 10 panels designed to interrogate metabolic pathways along with ionic, osmotic and pH effects, and 10 panels to assess the sensitivity to various antimicrobials with different mechanisms of action.
PM1 – PM10 (Metabolic tests for bacteria and fungi)
The OmniLog™ software contains a suite of algorithms that work in conjunction with the OmniLog™ and the Phenotype MicroArray™ panels to automate incubation of up to 50 microplates at a user-specified temperature with continuous collection of colorimetric assay data over time. Data analysis programs allow for display of kinetic data, management and analysis of data, and facilitates export in a variety of raw and processed forms.
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Software Functions
File Management/Kinetic/Parametric Analysis
Parametric Analysis
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