Examine environmental community diversity with Biolog EcoPlates™
Biolog EcoPlates provide a sensitive and reliable index of environmental change. This approach, called community-level physiological profiling (CLPP), is effective at distinguishing spatial and temporal changes in the metabolism of microbial communities.
Microbial communities provide useful information about environmental change. Microorganisms are present in virtually all environments and are typically the first organisms to react to chemical and physical changes in the environment. Changes in microbial communities are often a precursor to changes in the health and viability of the environment as a whole.
31 carbon assays in triplicate
EcoPlates contain 3 repeated sets of 31 carbon sources and employ a tetrazolium redox dye as an indicator of microbial metabolism. As microbes utilise the carbon sources they respire and the tetrazolium reporter dye is reduced to form a visible purple colour. Communities of microorganisms will exhibit a characteristic reaction pattern, a metabolic fingerprint, that reflects the metabolic properties of the community.
- Measure the metabolism of 31 carbon sources per assay
- Each assay panel tests in triplicate
- Simple colorimetric readout
- Readable with any microplate reader
STEP 1: Prepare and pipet assay mixture containing cell permeabilising buffer and redox dye into appropriate wells.
STEP 2: Start the assays by adding 2x cell suspension to all wells.
STEP 3: Load the MicroPlate into the OmniLog® for kinetic reading of the rate of purple colour formation.
Biolog MitoPlate S-1 and I-1 Protocol (10-17-2018) from Biolog on Vimeo.
- An essential tool in your milk quality-assurance program.
- MitoPlates are preloaded with 96 tests ready for use
- Plates designed to measure effects of substrates and inhibitors on mitochondrial function
- Easy, robust protocols with any cell type – adherent or suspension cells, transformed cell lines or primary cells
- Assays need only 20,000 to 40,000 cells per well
- Novel tetrazolium dye chemistry provides a terminal electron acceptor in easy to read colorimetric assays
- OmniLog instrument provides automated temperature controlled incubation and kinetic reading of multiple plates (50 plates at 15 min, or 16 at 5 min intervals)